On 10 November 1922, Pope Pius XI recognized Lithuania de jure.
In 1940, Lithuanian diplomatic representation in the Holy See was not closed as the Holy See did not recognize the annexation of Lithuania.
On 30 September 1991, diplomatic relations were re-established.
From 1992 to 2004, Kazys Lozoraitis served as the first Ambassador of Lithuania to the Holy See after the re-establishment of independence.
From 1992 to 1997, Justo Mullor Garcia served as the first Apostolic Nuncio to Lithuania.